Automated Multi-Layered Multiplex Assay Production with Inkjet Technology
Poster Feb 01, 2007
Susan Seaton, Duncan Hall and Howard Manning
This work reports on the production and exact reproducible nature of the Aj120 Inkjet Microarray Spotter when printing multiple droplet microarrays. This has lead to successful collaborations between Arrayjet and a number of scientific groups furthering the use of this novel method of non-contact printing within the microarray field, a few of which are highlighted here: cell lysate microarrays to develop new approaches in the diagnosis of cancer, antibody microarrays to detect and purify antigens, and “biochip” microarrays to detect chemicals and biologicals for homeland defence.
When there is a need to quickly analyze samples using a number of different PCR assays, it is likely that optimal conditions for each assay will not be the same. First, different assays often will require different annealing temperatures for their primers. In addition, amplicons may be designed to be of different lengths and therefore require varying durations of the extension step.READ MORE