Automated Nanolitre Hit Automated Nanolitre Hit - Picking using a Mosquito® X1 Low Volume Pipettor
Poster Mar 02, 2006
Joby Jenkins, Rob Lewis, Tristan Cope, Wayne Bowen
The system’s unique positive displacement pipetting technology allows it to pipette accurately and reproducibly throughout the 25 nL - 1.2 µL range, producing CVs of <10% at 25 nL with a cycle time of around 7 seconds. The mosquito X1 can be integrated with common plate handlers for vastly increased walk-away time.
We utilized paired synthetic crRNAs coupled with our synthetic tracrRNA in cells transduced with lentiviral Cas9 to perform a functional knockout on hsa-miR-221. This three-part system (crRNA, tracrRNA and Cas9) has demonstrated efficient gene editing when used with only one guide RNA, but the goal was to use two crRNAs to remove the entire stem-loop.READ MORE
During early drug discovery, the study of metabolism plays an essential role in determining which drug candidates move forward into development and later stages. As an alternative to traditional Data Dependent Acquisition (DDA), the use of MSE/All Ions Fragmentation (AIF) has become common in metabolite identification workflows for the analysis of metabolic hot spots. Here we present a solution for analysis of MSE/AlF in metID studies.READ MORE