Automated Quantitative Analysis of Ethanol, Glucose and NADH in the UV/VIS and Short NIR
Poster Oct 11, 2013
There is a variety of probes and analytical techniques on the market for monitoring oxygen concentration, pH value, temperature or optical density. But similar automated analytical techniques are currently not available for a continuous measurement of other biotechnologically important substances like glucose, ethanol and NADH. In order to measure these substances directly and automated in the bioreactor, a suitable measurement system is required. For this purpose, in-line absorbance measurements have already been performed in the NIR and MIR. Advantages of these wavelength ranges over the UV/VIS are the stronger absorbance. However, the wavelength range from 400nm to 1000nm is also exciting due to the lower interfering water absorption and the low cost measurement technology based on silicon. Besides it is interesting due to the possible fluorescence of NADH.
The aim of this work will be to clarify whether it is possible to build up a system for automated in-line measurement of bioprocess parameters and to develop a calibration model for a prediction of concentrations via multivariate chemometric algorithms for UV/VIS and short NIR.
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The determination of geographical origin of wine is gaining increased interest by researchers and federal agencies around the world, partially due to increased fraud with regards to place of origin labelling. For wine, multi-elemental profiling of macro, micro, and trace elements has been proposed for determination of authenticity. Commercial wines from different wineries in 5 different neighborhoods within one AVA show characteristic elemental fingerprints. Macro, micro and trace elements as well as elemental ratios contribute to the observed separation, indicating the involvement of multiple factors and underlying mechanisms, including location and soil composition, elemental uptake by vine and rootstock, viticulture and nutrient management, water sources, and small differences in the different wineries.READ MORE
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The analysis of the elemental composition of whiskies provides a host of important information including sample origin and understanding how different whiskey styles are caused by processing equipment and raw materials. Preliminary data analysis of the whiskies showed that element compositions could possibly be used to differentiate samples based on age, type, and region.READ MORE