Automated Quantitative Analysis of Ethanol, Glucose and NADH in the UV/VIS and Short NIR
Poster Jul 02, 2013
Linda Grober, Ulla Wenzel, Princz Sascha, Rudolf Miller and Martin Hessling
There is a variety of probes and analytical techniques on the market for monitoring oxygen concentration, pH value, temperature or optical density. But similar automated analytical techniques are currently not available for a continuous measurement of other biotechnologically important substances like glucose, ethanol and NADH. In order to measure these substances directly and automated in the bioreactor, a suitable measurement system is required. For this purpose, in-line absorbance measurements have already been performed in the NIR and MIR. Advantages of these wavelength ranges over the UV/VIS are the stronger absorbance. However, the wavelength range from 400nm to 1000nm is also exciting due to the lower interfering water absorption and the low cost measurement technology based on silicon. Besides it is interesting due to the possible fluorescence of NADH.
The aim of this work will be to clarify whether it is possible to build up a system for automated in-line measurement of bioprocess parameters and to develop a calibration model for a prediction of concentrations via multivariate chemometric algorithms for UV/VIS and short NIR.
Elemental profiles of whiskies allow differentiation by type and region by inductively coupled plasma –optical emission spectroscopy (ICP-OES)Poster
The analysis of the elemental composition of whiskies provides a host of important information including sample origin and understanding how different whiskey styles are caused by processing equipment and raw materials. Preliminary data analysis of the whiskies showed that element compositions could possibly be used to differentiate samples based on age, type, and region.READ MORE
Determination of the Fat Content Profile of different Chocolate Products using an Automated Workflow for the Generation of Fatty Acid Methyl Esters (FAME)Poster
The method allows for automated determination of total fat content and quantitative analysis of saturated and unsaturated cis- and trans- fatty acids of different chocolate products.READ MORE
Extraction of Analytes of Toxicolgical Interest from Plasma with Enhanced Matrix Removal-Lipid MaterialPoster
Extraction of Analytes of Toxicolgical Interest from Plasma with Enhanced Matrix Removal-Lipid (EMR-Lipid) Material.READ MORE