Automated Solid-Phase Extraction of Oraganochlorine Pesticides from Drinking Water
Poster Jul 30, 2014
Frans Schoustsen and Pranathi R Perati
Organochlorine pesticides (OCP) are a class of chemicals that were used to control insect pests since the 1940s. The use of OCPs was banned in the later part of last century due to their longevity, a trait that made them effective for long term pest control, but also increased concerns of potential health outcomes such as cancer in humans and ecosystem disruption.
Pesticides are regulated in the U.S. by the Environmental Protection Agency (EPA) under the Federal Insecticide, Fungicide, and Rodenticide Act (FIFRA). Some states also regulate pesticides under FIFRA, in a more restrictive manner than the EPA. The Stockholm Convention on Persistent Organic Pollutants, a UN treaty, has established global bans on several organochlorine pesticides including DDT, hexachlorobenzene, pentachlorobenzene, chlordane, dieldrin, endrin, heptachlor, mirex, toxaphene, hexachlorocyclohexane (alpha-HCH, beta-HCH, and gamma-HCH (lindane)), and chlordecone.
OCPs can be highly toxic, are hydrophobic, lipophilic, and extremely stable. Once in the air, water, and soil, they are subject to global deposition processes and bioaccumulation in the food chain. Diet is the main source of human exposure, primarily through food consumption where OCPs have bioaccumulated.
In this study twenty chlorinated pesticides were extracted from drinking water using a Thermo Scientific™ Dionex™ AutoTrace™ 280 Solid-Phase Extraction instrument and the Thermo Scientific™ Dionex™ SolEx™ C 18 cartridges. The recoveries were compared to the traditional liquid-liquid extraction method (LLE). Analysis for both sample preparation methods was performed by gas chromatography with Electron Capture Detection (GC-ECD)
High Speed Analysis: Combining Fast GC with Time-of-Flight Mass Spectrometry for Complex Sample Analysis in Under One MinutePoster
The demand for more efficiency and productivity in the laboratory has led to applications using Fast GC-MS methods for routine analysis in a wide range of fields.READ MORE
Screening of ALDH2 Interactome by Combined Immunoprecipitation and Mass SpectrometryPoster
ALDH2 may have an unidentified protein interaction network, which would help elucidate its importance in various cellular functions. The combination of immunoprecipitation and mass spectrometry will give us insights that could not be easily attained otherwise.READ MORE
Nitrogen metabolism in Mycobacterium tuberculosis: a systems-based approachPoster
Previous studies suggested that Mycobacterium tuberculosis obtains nitrogen from a diverse range of intracellular nutrients including amino acids. Here, we use a novel system’s based three-pronged approach to define pathways for uptake and assimilation of nitrogen.READ MORE
Comments | 0 ADD COMMENT
Asia Pacific Congress on Probiotics, Prebiotics and Nutrition
Oct 15 - Oct 16, 2018
15th Symposium on the Practical Applications of Mass Spectrometry in the Biotechnology Industry
Sep 09 - Sep 12, 2018
CE in the Biotechnology & Pharmaceutical Industries: 20th Symposium on the Practical Applications for the Analysis of Proteins, Nucleotides & Small Molecules
Sep 09 - Sep 12, 2018