AUTOMATED WORKFLOW FOR THE DETERMINATION OF FATTY ACID METHYL ESTERS (FAME)
Poster May 19, 2014
Beat Schilling1, Reto Bolliger2, Guenter Boehm2
The analysis of oils, fat and fat containing food via fatty acid methyl esters (FAME) is a common task in governmental, quality control (QC) or contract research laboratories (CRO). In most cases the samples are processed manually, which is labor intensive and exposes the lab personnel to potentially hazardous chemicals [1,2].
This poster presents a fully automated workflow using a Workstation with robotic tool change (RTC, Fig. 1) based on a method using sodium methoxide in methanol as reactant . The workflow improves process safety, optimizes throughput and minimizes handling errors. The PALworkstation was equipped with a Dilutor to dispense the liquids for the reactions, the extraction and the cleaning steps, a Vortex module to provide fast mixing and extraction and a tool for a 10 μl syringe to inject the sample into the GC. The software of the workstation allows overlapped sample processing, which increases sample throughput.
The method allows the determination of the total fat content, quantitative analysis of saturated and unsaturated cis- and trans-fatty acids. Three internal standards are used to control extraction, transesterification and undesired saponification. The method was applied to a number of different vegetable oils and water containing animal fats such as butter, cheese and salami.
The Use of Pyrolysis/GCMS and Newly Developed Libraries to assist in Characterizing Complex Polymeric SamplesPoster
Pyrolysis-GC/MS is an effective tool in introducing and analyzing polymers. But since the polymer must be broken down to volatile segments in order to be sent to the GC, identification by the Mass Spec does not look at the original polymer, but only the fragments. This poster will look at a unique library designed to work with the existing NIST library, however contains averaged spectra of pyrolyzed polymers so it can be used to directly identify the original polymer sample.READ MORE
Application of Solid Phase Micro Extraction (SPME) and On‐Fiber Derivatization to Detect Precursors of Biomolecules in the study of Proto‐metabolismPoster
In the studies of origins of life, plausible synthetic reaction pathways to form biological relevant molecules, or building blocks of proto‐bilayer lipids, protein, RNA, and DNA in the primitive environment have been investigated intensively.READ MORE
A New Tool for the Automated Sample Preparation of Whole Blood Samples by LC-MS using a Commercial AutosamplerPoster
Automated sample preparation reduces the costs per sample and minimizes sample handling errors. Usually expensive and highly specialized pipetting robots are used. However, most of these systems are not designed with a direct interface for LC-MS applications. In addition common pipetting systems are not optimized for smaller scale sample series. Here we present a new tool for liquid handling of whole blood samples and direct sample injection.READ MORE