AUTOMATED WORKFLOW FOR THE DETERMINATION OF FATTY ACID METHYL ESTERS (FAME)
Poster May 19, 2014
Beat Schilling1, Reto Bolliger2, Guenter Boehm2
The analysis of oils, fat and fat containing food via fatty acid methyl esters (FAME) is a common task in governmental, quality control (QC) or contract research laboratories (CRO). In most cases the samples are processed manually, which is labor intensive and exposes the lab personnel to potentially hazardous chemicals [1,2].
This poster presents a fully automated workflow using a Workstation with robotic tool change (RTC, Fig. 1) based on a method using sodium methoxide in methanol as reactant . The workflow improves process safety, optimizes throughput and minimizes handling errors. The PALworkstation was equipped with a Dilutor to dispense the liquids for the reactions, the extraction and the cleaning steps, a Vortex module to provide fast mixing and extraction and a tool for a 10 μl syringe to inject the sample into the GC. The software of the workstation allows overlapped sample processing, which increases sample throughput.
The method allows the determination of the total fat content, quantitative analysis of saturated and unsaturated cis- and trans-fatty acids. Three internal standards are used to control extraction, transesterification and undesired saponification. The method was applied to a number of different vegetable oils and water containing animal fats such as butter, cheese and salami.
Proteomics and Substrate Based MS Imaging of Xenobiotic Metabolising Enzymes in Ex Vivo Human Skin and a Human Living Skin Equivalent ModelPoster
Untargeted proteomics analysis showed that human skin and a commercially available living skin equivalent model exhibit a similar distribution of xenobiotic metabolising enzymes. A new technique, substrate based mass spectrometry imaging (SB-MSI) was developed during this study.READ MORE
Development and Assessment of Non-Infected and Infected Skin Models Using MALDI-MSIPoster
Infections have detrimental effects on wound healing leading to chronic wound formation. The use of labskin,a living skin model, in combination with mass spectrometry imaging, provides a good model for assessing wound healing factors.READ MORE
Overcoming the Challenges of Analysing Ionic Polar Pesticides in FoodPoster
Here, the novel application of the Waters’ Torus DEA column is presented, showing the improved performance of a UPLC-MS/MS method for the underivatised
analysis of glyphosate and a selection of other anionic pesticides.