Automation of a Generic Fluorescence Methyltransferase Activity Assay
Poster Feb 16, 2012
X. Amouretti, P. Brescia, P. Banks, G. Prescott, Meera Kumar
Methylation is known to be a ubiquitous covalent modification involved in regulation of a diverse range of biomolecules. Histone methyltransferases (HMTs) are of particular interest as drug targets as histone methylation is linked to certain disease states, including a wide variety of cancer types. A high-throughput screening (HTS)-ready, universal methyltransferase activity assay was recently developed based on competitive fluorescent polarization immunodetection of AMP, formed from the methyltransferase (MT) reaction product S-adenosylhomocysteine in a dual enzyme coupling step. This work demonstrates automation of the assay in a 384-well format suitable for HTS.
Histone Deacetylase 6 (HDAC6) As A Therapeutic Target in Chronic Lymphocytic LeukemiaPoster
This study investigates the role of histone deacetylase 6 (HDAC6) in chronic lymphocytic leukemia and establishes it as a novel therapeutic target for the treatment of this disease.READ MORE
Inhibition of The Auto-inflammation Suppressor Protein ISG15 Triggers Preeclampsia by Blocking Trophoblast Migration and InvasionPoster
In summary, ISG15 expression levels are crucial for trophoblast morphology and function (migration/invasion). By blocking trophoblast invasion, reduced ISG15 levels could contribute to impaired spiral artery transformation that reduces utero-placental blood flow in preeclampsia. Thus, agents inducing ISG15 expression are likely to be therapeutic in preeclampsia.
Magnetic-microcontact printing based ECM nano-patterning allows homogeneous controlling of cell growth and behaviorPoster
Magnetic – microcontact printing of extracellular matrix proteins (ECM) results in cell microarrays (CMA) showing homogeneous cell growth and attachment on defined patterns. This greatly reduces bias in the analysis of cell responses to toxins, drugs or other stimuli.READ MORE