Blood Transcriptomic Diagnosis of Active TB with Five Genes
Poster Sep 20, 2016
JK Roe1*, N Thomas1, E Gil1, K Best1, E Tsaliki1, S Morris-Jones2, S Stafford1, N Simpson1, KD Witt3, B Chain1, R Miller4, A Martineau3, M Noursadeghi1
Confirming the diagnosis of active tuberculosis (TB) requires samples from the site of disease. Peripheral blood transcriptional profiling offers a potential alternative. Multiparametric blood transcriptomic signatures of TB have been described as potential diagnostic tests.
When there is a need to quickly analyze samples using a number of different PCR assays, it is likely that optimal conditions for each assay will not be the same. First, different assays often will require different annealing temperatures for their primers. In addition, amplicons may be designed to be of different lengths and therefore require varying durations of the extension step.READ MORE