Busted! First Detection of Steroid Hormones in Pacific Walrus Bones
Poster Jan 19, 2015
Charapata P, Horstmann L, and Misarti N
The Pacific walrus (Odobenus rosmarus divergens) is a candidate for the Endangered Species List, because climate warming could affect habitat use and food web structure. With the current change of the Arctic ecosystem, it is unclear how walruses will respond to these possible stressors. In this novel study, steroid hormones (i.e., progesterone, testosterone, estradiol, estriol, and cortisol) were extracted from archeological (927-3,585 years old), historical (34-81 years old), and modern (year 2014 samples) bones to determine if hormones could be detected in bones of various ages. Lipids were removed and subsequently steroid hormones, from powdered bone using a methanol extraction procedure. Hormone levels were analyzed and validated by liquid chromatography triple quadrupole mass spectrometry (LC-QQQ MS). Progesterone, testosterone, cortisol, and estriol were detected, while estradiol concentrations were below the detection limit (<1 ng/g) in all samples. We were able to detect cortisol in archaeological (n=1 of 7, 3.71 ng/g), historical (n=3 of 52 mean= 4.07 ng/g), and modern (n=1 of 10, 6.56 ng/g) bone samples. Progesterone was detected in archaeological (n=4 of 7, mean= 1.47 ng/g), historical (n=51 of 52, mean=14.76 ng/g), and modern (n=8 of 10, mean=10.08) bone samples. Testosterone was detected in archaeological (n=3 of 7, mean= 0.24 ng/g), historical (n=26 of 52, mean=0.98 ng/g), and modern (n=6 of 10, mean=1.03 ng/g). Estriol was only detected in archaeological (n=7 of 7, mean= 79.04 ng/g) bone samples. These results validate our method of steroid hormone extraction and are the first to address environmental interactions and physiological responses in historic and pre-historic walrus populations.
Identifying Non-Intentionally Added Substances (NIAS) in Food Contact MaterialsPoster
Concern about the safety of food products has increased dramatically with intentionally and non-intentionally added substances (NIAS) used in packaging being of particular interest. APGC and high resolution MS together with a workflow driven process was used to identify unknown compounds in packaging.READ MORE
Human exposure to cyanotoxins: Exploring in vitro detoxification using atmospheric cold plasma treatment to protect human healthPoster
Recently, there have been increases in freshwater harmful algal blooms. There is therefore a need to try and effectively detoxify drinking water contaminated with cyanotoxins to safeguard human health by use of new and novel techniques.READ MORE
Identifying Asthma-related Health Disparities Among Children in Hillsborough CountyPoster
Our project was to identify a geographic area demonstrating need for asthma interventions. By identifying different factors such as air quality, prevalence, asthma related hospitalizations and emergency department visits, we created a tool to identify and prioritize areas that could benefit from interventions like patient education and certification as an Asthma-Friendly School.