CAPILLARY ELECTROPHORESIS OF APIGENIN
Poster Aug 25, 2014
Paulina Furmaniak, Pawel Kubalczyk, Rafal Glowacki
Increasing interest in phytocompounds especially flavonoids is a result of their widespread occurrence in plant kingdom and wide range of biological activity. Apigenin (4,5,7-trihydroxyflavone), a naturally occurring plant flavone is a potent antioxidant that exhibits anti-inflammatory activities, is a scavenger of free radicals, and also prevents oxidation of vitamins C, E and glutathione. Apigenin exhibit some antitumor effects by inhibiting the tyrosine kinase activity of topoisomerase, angiogenesis and protecting against oxidative damage of DNA.
Capillary electrophoresis (CE) is a modern analytical technique which allows effective separation of charged particles in electric field. CE is considered as a green analytical technique due to its low reagent consumption, small sample volume and short analysis time. Unfortunately, important limitation on the application of capillary electrophoresis to biological samples is high limit of detection.
Very important role in the process of increasing the sensitivity of capillary electrophoresis play an analyte sweeping methods inside the capillary. One promising modality for sweeping the sample in the capillary is micellar electrokinetic chromatography (MEKC). The most suitable electrolyte for this separation was a buffer solution (pH 10.1) of 0.03 M sodium borate with addition of sodium dodecyl sulfate (0.01M) and acetonitrile (10%). Analysis of a complex matrix (plant extracts) requires precise step of sample preparation. The air-dried parsley leaves were powdered and several times extracted with methanol and ethyl acetate. The extract was in the last step centrifuged and the resulting supernatant was subjected to CE analysis. All separations were performed at 25C with detector set to measure peaks at 390 nm.
Using Elemental Analysis For Discrimination Of Pinot Noir Wines From Six Different Districts In An AvaPoster
The determination of geographical origin of wine is gaining increased interest by researchers and federal agencies around the world, partially due to increased fraud with regards to place of origin labelling. For wine, multi-elemental profiling of macro, micro, and trace elements has been proposed for determination of authenticity. Commercial wines from different wineries in 5 different neighborhoods within one AVA show characteristic elemental fingerprints. Macro, micro and trace elements as well as elemental ratios contribute to the observed separation, indicating the involvement of multiple factors and underlying mechanisms, including location and soil composition, elemental uptake by vine and rootstock, viticulture and nutrient management, water sources, and small differences in the different wineries.READ MORE
Fast arsenic speciation analysis of wines and rice with LC-ICP-QQQPoster
This method was designed in response to recent and proposed food standards, both international and national, that limit inorganic arsenic rather than total, organic, or individual arsenic species such as arsenite (AsIII) and arsenate (AsV). Analysis time is 10x faster than the current FDA regulatory method, increasing sample throughput, avoided spectral interferences and dramatically increased sensitivity. Validation data from two laboratories demonstrate the method’s accuracy and reproducibility of both wine and rice matrices in a single analytical batch.READ MORE
Elemental profiles of whiskies allow differentiation by type and region by inductively coupled plasma –optical emission spectroscopy (ICP-OES)Poster
The analysis of the elemental composition of whiskies provides a host of important information including sample origin and understanding how different whiskey styles are caused by processing equipment and raw materials. Preliminary data analysis of the whiskies showed that element compositions could possibly be used to differentiate samples based on age, type, and region.READ MORE