Cell Culture and Cell Analysis using the Real Architecture for 3D Tissue (RAFT™) Culture System
Poster Apr 26, 2016
Cecile Villemant1 , Sabine Schäpermeier2 , Stefanie Büsch2 , John Langer3 , Theresa D’Souza3 , Lubna Hussain3 , Grant Cameron1 , Volker Vogel2 , Jenny Schroeder2
Conventional in vitro assays are based on cells grown on two-dimensional (2D) substrates, which are not representative of a true in vivo cell environment. Three-dimensional (3D) cell culture methods, in contrast, allow cells to grow in structures more resembling the in vivo environment. Cells can develop cell-cell and cell-extracellular matrix (ECM) interactions in 3D. While 3D cultures more accurately resemble the in vivo environment, it might be difficult to analyze cells in 3D. However, this is not always the case. This poster explains how standard analysis techniques, like fluorescence microscopy, can be applied easily to RAFT™ 3D Cell Cultures. In addition, it will be shown how the RAFT™ Technology can be combined with transient transfection approaches using the Lonza 4D Nucleofector™ System.
Multiplexing cell-based assays is possible using 3D culture models that are larger and more complex than monolayers
Real-time detection methods to measure live or dead cells provide much flexibility for multiplexing
All multiplexed assay combinations should be verified using appropriate controls for each 3D cell culture model.
Basic fibroblast growth factor (bFGF) is widely used in vitro for the maintenance and stimulation of a variety of cells. However, use of native bFGF in cell biology is limited by the fact that bFGF rapidly degrades at physiological temperatures. We have addressed this problem with an engineered form of bFGF, named Heat Stable bFGF (HS bFGF), which is stable at 37 degrees Celsius.READ MORE