Cell Culture and Cell Analysis using the Real Architecture for 3D Tissue (RAFT™) Culture System
Poster Apr 26, 2016
Cecile Villemant1 , Sabine Schäpermeier2 , Stefanie Büsch2 , John Langer3 , Theresa D’Souza3 , Lubna Hussain3 , Grant Cameron1 , Volker Vogel2 , Jenny Schroeder2
Conventional in vitro assays are based on cells grown on two-dimensional (2D) substrates, which are not representative of a true in vivo cell environment. Three-dimensional (3D) cell culture methods, in contrast, allow cells to grow in structures more resembling the in vivo environment. Cells can develop cell-cell and cell-extracellular matrix (ECM) interactions in 3D. While 3D cultures more accurately resemble the in vivo environment, it might be difficult to analyze cells in 3D. However, this is not always the case. This poster explains how standard analysis techniques, like fluorescence microscopy, can be applied easily to RAFT™ 3D Cell Cultures. In addition, it will be shown how the RAFT™ Technology can be combined with transient transfection approaches using the Lonza 4D Nucleofector™ System.
The nuclear receptors pregnane X receptor (PXR) and constitutive androstane receptor (CAR) are closely related transcription factors that regulate the expression of phase I (cytochrome P450s), phase II metabolizing enzymes and transporter genes in response to xenobiotics, including prescription drugs.READ MORE