Cell Culture and Cell Analysis using the Real Architecture for 3D Tissue (RAFT™) Culture System

Conventional in vitro assays are based on cells grown on two-dimensional (2D) substrates, which are not representative of a true in vivo cell environment. Three-dimensional (3D) cell culture methods, in contrast, allow cells to grow in structures more resembling the in vivo environment. Cells can develop cell-cell and cell-extracellular matrix (ECM) interactions in 3D. While 3D cultures more accurately resemble the in vivo environment, it might be difficult to analyze cells in 3D. However, this is not always the case. This poster explains how standard analysis techniques, like fluorescence microscopy, can be applied easily to RAFT™ 3D Cell Cultures. In addition, it will be shown how the RAFT™ Technology can be combined with transient transfection approaches using the Lonza 4D Nucleofector™ System.