Characterisation & Potential Applications of Human iPS Cell Derived Neural Progenitor Cells
Poster Sep 02, 2014
Wei J1., Gibbons G1., Lopez Alcantara S2., Dale T2., González Rueda A3., Paulsen O3. & Cox C1.
In 2012, Shinya Yamanaka was awarded a Nobel Prize for demonstrating
that adult cells can be reprogrammed into induced pluripotent stem
cells (iPS cells) using defined factors- Oct3/4, KLF4, Sox2 and c-Myc1
in mouse in 2006 and human in 2007. iPS cells can be differentiated
into human neural progenitor cells (hNPCs) and cerebral cortical
neurons (hCCNs) from healthy donors and patients. Traditionally,
animal models have been utilised for disease research, however the
genetic and physiological differences between animal and humans means
these results are not always translatable to results in a human
system. By using iPS-derived human cells as an alternative system for
disease modelling and drug discovery, some of these issues can be
overcome. We characterised iPS cell-derived human neural progenitor
cells and their progeny produced using optimised methods to examine
their suitability to model neurobiology in a dish for use in numerous
applications including drug screening and toxicity testing.
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Multiplexing cell-based assays is possible using 3D culture models that are larger and more complex than monolayers
Real-time detection methods to measure live or dead cells provide much flexibility for multiplexing
All multiplexed assay combinations should be verified using appropriate controls for each 3D cell culture model.
Basic fibroblast growth factor (bFGF) is widely used in vitro for the maintenance and stimulation of a variety of cells. However, use of native bFGF in cell biology is limited by the fact that bFGF rapidly degrades at physiological temperatures. We have addressed this problem with an engineered form of bFGF, named Heat Stable bFGF (HS bFGF), which is stable at 37 degrees Celsius.READ MORE