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Poster

Characterization and Quantification of Essential Oils by GC and GCxGC with TOFMS and FID

Rectangle Image
Poster

Characterization and Quantification of Essential Oils by GC and GCxGC with TOFMS and FID

In this poster, GC and GC×GC data for essential oils are presented to demonstrate the benefits of a comprehensive second separation dimension. One-dimensional GC separations with long columns and slow temperature programs to chromatographically isolate each component are commonly paired with FID detection to determine quantitative Area % information for the components of an essential oil sample. Even with the long analysis time, typical in the industry, coelutions were observed in these data leading to some inaccuracies in the Area % values when single peaks were comprised of multiple analytes. With TOFMS and related deconvolution tools, many coelutions were mathematically resolved and relative area information was determined. Without calibration, the Area % values can be impacted by variations of analyte response with MS detection. The addition of a secondary separation dimension with GC×GC-FID chromatographically separated many of the coelutions in the second dimension and provided more accuracy to the Area % determinations. As this relieved the requirement for all analytes to be completely resolved in the primary separation dimension, a reduction in overall analysis time was achieved with a simultaneous improvement in the isolation of individual analytes. The structured nature of the 2D separation space and the distinct visual chromatograms also provided characterization capabilities of the essential oils. Analyte characteristics could be deduced based on an unknown analyte’s elution relative to the structured bands of analytes with similar functional groups and properties. The distinct visual chromatograms also provided improved chemical fingerprinting for rapid characterization and differential analysis of samples. Methods are reported and examples of these benefits are highlighted in this poster
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