Characterization of Protein and Protein Aggregates using Temperature-controlled Hollow-Fiber Flow-FFF

Hollow-Fiber Flow Field-Flow Fractionation (HF5) [1,2] is a subtechnique of conventional Asymmetrical Flow Field-flow fractionation (AF4). While AF4 uses a rectangular separation chan-nel, the separation in HF5 takes place in a tubular channel consisting of semi-permeable hollow fibers, which are packed in a respective cartridge. The HF5-cartridge can be used as a disposable in a conventional Postnova AF2000 AF4-system offering fast analysis times, sterile conditions as well as a very good reproducibility. Hence, HF5 represents an excellent fractionation system for e.g., pharmaceutical and biotechnological applications.