Characterizing GPCR Activation Using Automated Live Cell Imaging
Poster Jul 24, 2017
Joe Clayton and Peter Banks
G protein coupled receptor (GPCR)-mediated pathways are critical for cells to respond to intercellular and environmental cues, and are a major focus of drug discovery efforts, particularly for cancer treatment. The molecules that activate GPCRs, and the resulting signaling cascades triggered by associated G proteins, are diverse. Fluorescent dyes and biosensors can be used to monitor changes in second messenger levels, including Ca2+ and cyclic AMP (cAMP), in response to GPCR activation. Here we describe a live cell imaging based approach to detect GPCR activation using the Lionheart™ FX Automated Live Cell Imager and Gen5™ Microplate Reader and Imager Software. This method provides a large assay window and improved sensitivity over methods relying on total fluorescence intensity measurements. Dual in-line dispense tips enable addition of GPCR agonists with continuous monitoring of cellular response. Additionally, an image capture rate of up 20 frames per second enables characterization of rapid GPCR kinetics.
Extracorporeal shockwave therapy accelerates motor axon regeneration despite a phenotypically mismatched environmentPoster
A femoral nerve defect model was adapted for the evaluation of proregenerative effects of extracorporeal shockwave therapy (ESWT). Functional evaluation, histology and qRT-PCR data show differences between sensory and motor-derived nerve transplants and a pro-regenerative effect of ESWT. These data provide evidence for the clinical application of ESWT after autologous nerve transplantation as a novel non-invasive method.READ MORE
Severe Combined Immunodeficiency (SCID) caused by thymic aplasia due to a mutation in TBX1Poster
We report one of the first cases of TBX1 haploinsufficiency causing complete thymic aplasia and SCID.READ MORE