Chip’N’Fish : Novel Chemical Proteomics Platform for Rapid Identification of Small Molecule Drug Candidates Against Disease-Specific Targets
Poster Aug 31, 2005
György Dormán, László Puskás, László Hackler, Liliana Z. Fehér, Cesare Spadoni , Zsolt Lörincz, Tamás Jankovics, László Ürge and Ferenc Darvas
Identification and selection of disease-specific targets is one of the most critical steps in post-genomic drug-discovery. Over-expression of genes and their protein products is often the best starting point for dissecting the pathways that are responsible for the development of specific diseases. Such proteins often serve as molecular targets particularly in cancer, where small molecules are designed to inhibit directly oncogenic proteins that are mutated or overexpressed.
When there is a need to quickly analyze samples using a number of different PCR assays, it is likely that optimal conditions for each assay will not be the same. First, different assays often will require different annealing temperatures for their primers. In addition, amplicons may be designed to be of different lengths and therefore require varying durations of the extension step.READ MORE