ChIP-qPCR and qbasePLUS Jointly Identify a MYCN Activated miRNA Cluster in Cancer
Poster Sep 13, 2012
Barbara D’haene, Pieter Mestdagh, Daniel Muth, Frank Westerman, Frank Speleman and Jo Vandesompele
ChIP-qPCR starts with the treatment of intact nuclei with formaldehyde for the induction of protein-DNA crosslinks, trapping chromatin interacting with specific proteins (eg. transcription factors).
Despite the developments in conventional PCR, the complexity of multiplex Real Time PCR is still limited due to the lack of sufficient detection channels. To achieve high-end multiplexing capacity on standard Real Time PCR machines, Anapa Biotech has developed the MeltPlex® technology (see box on right).READ MORE