CiPA Phase 2 Study: validation of an automated microelectrode array (MEA) assay of hiPSC-derived cardiomyocyte electrophysiology for cardiac safety evaluation
Poster Mar 10, 2017
DC Millard, AM Nicolini, SA Chvatal, HB Hayes, M Clements, JD Ross
The Comprehensive in vitro Proarrhythmia Assay (CiPA), led by the US Food and Drug Administration, aims to reduce unwarranted drug attrition by improving preclinical cardiac safety evaluation. A key component of the proposed assay utilizes human stem cell derived cardiomyocytes (hSC-CMs) and microelectrode array (MEA) technology to evaluate the proarrhythmic risk of candidate compounds . An initial pilot study with 8 blinded compounds demonstrated reproducible results across 10 studies. The CiPA Phase 2 study with 28 blinded compounds was established to evaluate the ability of the CM-MEA assay to assess proarrhythmic risk. The increased scale of the CiPA Phase 2 study was used as a case study in the development and validation of an automated CM-MEA assay for cardiac safety evaluation.
The iCell Cardiomyocyte2 and Maestro APEX automated MEA workstation were used to evaluate 28 compounds of high, intermediate, and low proarrhythmic risk in a blinded study. The amplitude and field potential duration (FPD) were extracted from the raw field potential signal for each replicate and compound, providing information on the depolarization and repolarization of the cardiomyocyte monolayers, respectively. Additional beating characteristics, such as the beating rate, variability in beat rate, and occurrence of EADs were also included in the analysis. Corrected field potential duration (FPDc) was computed using the Fridericia correction.
These results support the use of hSC-CM and MEA technology for preclinical assessment of proarrhythmic risk within the proposed CiPA paradigm, and, more generally, demonstrate that automation of the CM-MEA assay can achieve high reliability and throughput for cardiac risk assessment in vitro.
Analysis of the Effect of Aggregated β-Amyloid on Cellular Signaling Pathways Critical for Memory in Alzheimer’s DiseasePoster
Here we evaluate the ability to detect changes in phosphorylation levels of ERK and CREB following treatment with Aβ using the SH-SY5Y neuroblastoma cell line.READ MORE
Bioluminescent Kinase Profiling Systems For Characterizing Small Molecule Kinase InhibitorsPoster
In order to profile compounds against a broad panel of kinases, in-house profiling requires rigorous kinase assay development.READ MORE
MEG Resting State Functional Connectivity and Network Topology in Dyslexia Related GenotypePoster
The preliminary results from this first MEG genetics study of dyslexia indicate differences in RSFC and MST networktopology (Degree) related to PCSK6 expression within the dyslexic population.READ MORE