Cloning And Purification of a Putative Repressor Protein from Arthrobacter nicotinovorans Pao1
Poster Oct 11, 2013
Besides the well-characterized pathway for nicotine degradation (Brandsch, 2006), pAO1 carries a gene cluster of a hypothetical pathway for carbohydrate utilization. This cluster consists of ORFs of a transcriptional regulator, of a sugar ABC-transporter and of several putative dehydrogenases and oxidoreductases. Previously, we established that the pAO1 orf39 gene encodes an aldehyde-dehydrogenase (Mihasan, 2010) and orf40 encodes an sugar dehydrogenase. Here we focus on further characterization the ORF32 protein and elucidation of its possible role in the cell. By cloning the gene in the plasmid vector pH6EX3, we were able to express it as a recombinant His-tagged protein and to easily purify it to homogenity.
Application of Solid Phase Micro Extraction (SPME) and On‐Fiber Derivatization to Detect Precursors of Biomolecules in the study of Proto‐metabolismPoster
In the studies of origins of life, plausible synthetic reaction pathways to form biological relevant molecules, or building blocks of proto‐bilayer lipids, protein, RNA, and DNA in the primitive environment have been investigated intensively.READ MORE
A Tissue-based Proteomic Study of VEGFR2 in Human Term PlacentasPoster
VEGFR2 is the main regulator of placental angiogenesis and at term is localized in endothelial cells (EC) of the villous vasculature. VEGFR2 immunoprecipitation (IP) of membrane proteins, extracted from the fetal compartment, isolated 30 proteins that were identified by proteomic analysis.READ MORE