Cloning And Purification of a Putative Repressor Protein from Arthrobacter nicotinovorans Pao1
Poster Jul 24, 2013
Marius Mihasan, Marius Stefan, Hritcu Lucian, Vlad Artenie
Besides the well-characterized pathway for nicotine degradation (Brandsch, 2006), pAO1 carries a gene cluster of a hypothetical pathway for carbohydrate utilization. This cluster consists of ORFs of a transcriptional regulator, of a sugar ABC-transporter and of several putative dehydrogenases and oxidoreductases. Previously, we established that the pAO1 orf39 gene encodes an aldehyde-dehydrogenase (Mihasan, 2010) and orf40 encodes an sugar dehydrogenase. Here we focus on further characterization the ORF32 protein and elucidation of its possible role in the cell. By cloning the gene in the plasmid vector pH6EX3, we were able to express it as a recombinant His-tagged protein and to easily purify it to homogenity.
Identifying Non-Intentionally Added Substances (NIAS) in Food Contact MaterialsPoster
Concern about the safety of food products has increased dramatically with intentionally and non-intentionally added substances (NIAS) used in packaging being of particular interest. APGC and high resolution MS together with a workflow driven process was used to identify unknown compounds in packaging.READ MORE
Human exposure to cyanotoxins: Exploring in vitro detoxification using atmospheric cold plasma treatment to protect human healthPoster
Recently, there have been increases in freshwater harmful algal blooms. There is therefore a need to try and effectively detoxify drinking water contaminated with cyanotoxins to safeguard human health by use of new and novel techniques.READ MORE
Psychiatric Risk Gene Cacna1c and Early Life Stress: Potential Gene-Environment interactions?Poster
Early life stress (ELS) is highly associated with development of psychopathology
and mood disorders in adulthood. Genetic studies have identified variation in the gene calcium voltage-gated channel subunit alpha1C (CACNA1C) to increase risk for several psychiatric disorders. This poster assessed the expression of Cacna1c following prepubertal stress.