Combining low and high volume liquid handling capabilities for ADME screening
Poster Jan 18, 2014
Joby Jenkins, Kevin Moore, Stephen Fowler, Pascal Schenk
Current pipetting technologies allow flexible pipetting only down to ~1 μl with sufficient precision and accuracy. In addition, volumes > 0.5% of DMSO, used as a solvent for compound collections, can inhibit enzyme activities.
To allow assays to be performed faster and using less reagents a fast, high quality nanolitre dispensing system is required.
In this study we demonstrate the integration of two liquid handlers to extend the volume dispensing range creating low-volume assay-ready plates with high accuracy and precision. This was then successfully applied to a CYP inhibition assay.
mosquito HTS’s ability to miniaturize assays means much smaller volumes, resulting in saving on resources such as buffers, assay kits, radio-ligands, and enzymes.
In addition, this integration could reduce the volume of DMSO waste and decrease the amount of valuable compound used by up to 16-fold.
We found a distinct subpopulation of Tregs within BMSCs. Tregs and BMSCs in co-culture conferred neuroprotection that varied in a dose-dependent manner. Tregs minimized stem cell production of IL-6, a pro-inflammatory cytokine, and inhibited BMSC secretion of FGF-beta, a cytokine related to BMSC proliferation and differentiation. The ratio of Tregs found natively in BMSCs is optimally adapted to provide the maximum neuroprotective benefit of stem cell treatment after ischemic stroke.READ MORE
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