Combining low and high volume liquid handling capabilities for ADME screening
Current pipetting technologies allow flexible pipetting only down to ~1 μl with sufficient precision and accuracy. In addition, volumes > 0.5% of DMSO, used as a solvent for compound collections, can inhibit enzyme activities.
To allow assays to be performed faster and using less reagents a fast, high quality nanolitre dispensing system is required.
In this study we demonstrate the integration of two liquid handlers to extend the volume dispensing range creating low-volume assay-ready plates with high accuracy and precision. This was then successfully applied to a CYP inhibition assay.
mosquito HTS’s ability to miniaturize assays means much smaller volumes, resulting in saving on resources such as buffers, assay kits, radio-ligands, and enzymes.
In addition, this integration could reduce the volume of DMSO waste and decrease the amount of valuable compound used by up to 16-fold.
To allow assays to be performed faster and using less reagents a fast, high quality nanolitre dispensing system is required.
In this study we demonstrate the integration of two liquid handlers to extend the volume dispensing range creating low-volume assay-ready plates with high accuracy and precision. This was then successfully applied to a CYP inhibition assay.
mosquito HTS’s ability to miniaturize assays means much smaller volumes, resulting in saving on resources such as buffers, assay kits, radio-ligands, and enzymes.
In addition, this integration could reduce the volume of DMSO waste and decrease the amount of valuable compound used by up to 16-fold.
