Erica Ginsburg, Umer Khan, Phillip Pham, Anmol Kundlas, Simran Gill
Epidemiological data and recent scientific publications provide support for the neuroprotective effects of coffee against AD(1). The proposed mechanisms of therapeutic benefits include inhibition of an enzyme known as alpha secretase which results in lower levels of Amyloid beta plaques and increase in a hematopoietic factor known as GCSF(2). The aim of this study is to find the amount of coffee and type of coffee which ensures cell survival while reducing amyloid beta levels. N2APP are neuroblastoma cells from the APPsw mouse model with mutated genes for developing Alzheimer’s disease. N2APP cells can be treated
with various drugs and the cytotoxicity of these drugs on their neurons can be measured using MTT assay. The survival rate of cells at various concentrations of coffee treatment can be quantified. 24 hours after drug treatment, the supernatant was collected, and amyloid beta levels were measured using ELISA. The data in this study is based on treatment of N2APP cells with Instant coffee and common commercial coffee solutions.
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Download this poster to learn how a comprehensive MALDI mass spectrometry (MALDI-IMS) approach to formalin-fixed paraffin-embedded (FFPE) tissue samples was developed to study cardiovascular pathology.
The nuclear receptors pregnane X receptor (PXR) and constitutive androstane receptor (CAR) are closely related transcription factors that regulate the expression of phase I (cytochrome P450s), phase II metabolizing enzymes and transporter genes in response to xenobiotics, including prescription drugs.