Comparison of Fluorescence Lifetime and Fluorescence Intensity Readouts using a Homogeneous Protease-assay
Poster Oct 14, 2005
Joachim Janda, Michael Busch, Christoph Wenger and Ralf Thiericke
In this work two measurement parameters are compared, the fluorescence intensity (FI) and fluorescence lifetime (FLT), which could be acquired efficiently in a multi parameter readout from one and the same measurement.
The biochemical reaction being investigated consists of a fluorescent labelled enzyme-substrate (case in as a biopolymer carrying BODIPY FL), which is cleaved by the protease a-chymotrypsin.
In the beginning of the assay the fluorescence signal is internally quenched as a result of energy transfer interactions among the BODIPY labels. The continuous fragmentation of the biopolymer backbone in consequence of enzymatic degradation leads to a rise of the fluorescence signal while the quenching effects decrease. In the assay this is expressed in an increase of both, intensity and lifetime.
Psychiatric Risk Gene Cacna1c and Early Life Stress: Potential Gene-Environment interactions?Poster
Early life stress (ELS) is highly associated with development of psychopathology
and mood disorders in adulthood. Genetic studies have identified variation in the gene calcium voltage-gated channel subunit alpha1C (CACNA1C) to increase risk for several psychiatric disorders. This poster assessed the expression of Cacna1c following prepubertal stress.
Novel Role of the Innate Immune DNA Sensor IFI16 (Interferon Gamma Inducible Protein 16) as a Major Epigenetic Modulator During KSHV Infection and Lytic ReactivationPoster
Studies have shown that IFI16 acts as an antiviral restriction factor against a number of DNA viruses, by inhibiting viral replication or transcription through epigenetic modifications. However, till date, no specific epigenetic function of IFI16 has been identified. Here, we have discovered that IFI16 recruits two histone methyltransferases on the KSHV episome leading to altered Histone H3K9 methylation, thus regulating its lifecycle.READ MORE
A new method for generating arrayed RNAi screening tools for any organismPoster
RNA interference (RNAi) using small interfering RNAs (siRNAs) is an important technology for down-regulation of gene expression and a powerful tool to study cellular processes and pathways. Previously, large collections of siRNAs were available only for traditional experimental model systems, such as human and mouse, and predominantly provided as chemically synthesized libraries.READ MORE