Comparison of Fluorescence Lifetime and Fluorescence Intensity Readouts using a Homogeneous Protease-assay
Poster Oct 14, 2005
Joachim Janda, Michael Busch, Christoph Wenger and Ralf Thiericke
In this work two measurement parameters are compared, the fluorescence intensity (FI) and fluorescence lifetime (FLT), which could be acquired efficiently in a multi parameter readout from one and the same measurement.
The biochemical reaction being investigated consists of a fluorescent labelled enzyme-substrate (case in as a biopolymer carrying BODIPY FL), which is cleaved by the protease a-chymotrypsin.
In the beginning of the assay the fluorescence signal is internally quenched as a result of energy transfer interactions among the BODIPY labels. The continuous fragmentation of the biopolymer backbone in consequence of enzymatic degradation leads to a rise of the fluorescence signal while the quenching effects decrease. In the assay this is expressed in an increase of both, intensity and lifetime.
Characterization of a Type 2 diabetes-associated islet-specific enhancer cluster in STARD10 by genome editing of EndoC-βH1 cellsPoster
Genome-wide association studies (GWAS) have identified more than 100 genetic loci associated with type 2 diabetes. The majority of these are located in the intergenic or intragenic regions suggesting that the implicated variants may alter chromatin conformation. This, in turn, is likely to influence the expression of nearby or more remotely located genes to alter beta cell function. At present, however, detailed molecular and functional analyses are still lacking for most of these variants. We recently analysed one of these loci and mapped five causal variants in an islet-specific enhancer cluster within the STARD10 gene locus. Here, we aimed to understand how these causal variants influence b-cell function by alteration of the chromatin structure of enhancer clusterREAD MORE
P450 Induction in Cryopreserved Hepatocytes from PXR and CAR Nuclear Receptor Knock-out RatsPoster
The nuclear receptors pregnane X receptor (PXR) and constitutive androstane receptor (CAR) are closely related transcription factors that regulate the expression of phase I (cytochrome P450s), phase II metabolizing enzymes and transporter genes in response to xenobiotics, including prescription drugs.READ MORE
Psychiatric Risk Gene Cacna1c and Early Life Stress: Potential Gene-Environment interactions?Poster
Early life stress (ELS) is highly associated with development of psychopathology
and mood disorders in adulthood. Genetic studies have identified variation in the gene calcium voltage-gated channel subunit alpha1C (CACNA1C) to increase risk for several psychiatric disorders. This poster assessed the expression of Cacna1c following prepubertal stress.