Continuous Collection of Stem Cells from a Human Placenta Perfusion Co-Culture
Poster Mar 16, 2015
John J.S. Cadwell & James C. Hardy
The presence of smaller cells in the ECS harvests, OCT3/4 phenotype and the highly proliferative nature of this harvest when placed into flasks suggests the presence of very small embryonic-like stem cells. The hollow fiber cell culture environment is clearly different than flask culture. Three dimensional structures were formed on the surface of the fibers. Cytokine concentration and cell to cell interactions may play a role in their formation. When harvested cells were placed into flasks, the different cell culture environment induced these cells to differentiate into mesenchymal stem cells and generate spheroids. The recapitulation of the placental construct may represent a unique source for continuous culture and collection of stem cells. Hollow fiber bioreactors represent a different cell culture environment with resultant differences in cell phenotype from flask culture. These experiments demonstrate the capability of hollow fiber bioreactors to support continuous production of certain stem cell types in a single use system. Larger scale systems have the potential to produce stem cells on a continuous basis and meet the criteria for their bio-manufacturing in a single-use system on a clinical scale.
Multiplexing cell-based assays is possible using 3D culture models that are larger and more complex than monolayers
Real-time detection methods to measure live or dead cells provide much flexibility for multiplexing
All multiplexed assay combinations should be verified using appropriate controls for each 3D cell culture model.
Basic fibroblast growth factor (bFGF) is widely used in vitro for the maintenance and stimulation of a variety of cells. However, use of native bFGF in cell biology is limited by the fact that bFGF rapidly degrades at physiological temperatures. We have addressed this problem with an engineered form of bFGF, named Heat Stable bFGF (HS bFGF), which is stable at 37 degrees Celsius.READ MORE