Cytosolic Delivery of Anticancer Drug Using Endosomolytic Peptide Bearing Nanoparticles Against Solid Tumor
Generally in case of targeted drug delivery to specific body organ or tissues, novel drug carriers such as liposomes, microsphere, nanoparticles, polymeric micelles and others are used and their endocytosis occurs by targeting cells. The two situations arise during endocytosis, (a) degradation of both polymeric carrier and drug may occur by endosomal content such as enzymes, pH condition and other related factors (b) expulsion of drug carrier system from target cells without drug release, and it can be prevented by lysing the endosomal layer. Hence, it is proposed to design polycynoacrylate NPs encapsulating anticancer drugs like doxorubicin. Encapsulation will lead higher drug loading in a system and then the NPs conjugated with transferrin on the surface which will target the NPs to tumor expressing transferrin receptors. Nanoparticles also contain a pH sensitive fusogenic peptide GALA which helps in endosomal scape of drug loaded NPs, (Yamada 2005).