Deciphering Regulatory Mechanisms in M. xanthus Using IsotopicRatio Outlier Analysis (IROA) for Metabolome-wide Quantitation
Poster Jun 15, 2014
Daniel Krug 1,2, Carsten Volz 1, Aiko Barsch 3, Chris Beecher 4, Felice de Jong 4, Rolf Müller 1,2
Myxobacteria are an important source of natural products often exhibiting potent biological activity and unusual modes-of-action . Their genomes - among the largest in prokaryotes - harbour intriguing numbers of secondary metabolite biosynthetic pathways, whereas the small-molecule products for many of these have yet to be discovered . Myxobacteria also devote an exceptionally high proportion of their genetic capacity to regulation, as exemplified by the genome of the model strain Myxococcus xanthus DK1622. Deciphering complex myxobacterial regulatory networks improves our ability to manipulate natural product yields and can facilitate the discovery of novel metabolites. Here, we present a comprehensive metabolomics study using comparative LC-hrMS profiling and IROA to quantify small-molecule readouts in response to induced overexpression of a transcriptional antiterminator in DK1622.
C1 Complement mediates human cord blood serum derived APP α-secretase cleavage activity in vitroPoster
Our results indicate that CBS contains proteins that promote α-secretase like enzymatic activity. LC-MS/MS analysis in CBSF and AgBSF revealed the presence of 142 proteins of which C1 subunits and alpha-2-macroglobulin showed significantly greater levels in αCBSF compared with αAgBSF. further study showed,C1 subunits can enhance sAPPα production and Aβ reduction in cell culture conditionREAD MORE
Fingerprinting the Terpene Profiles of Various Cannabis Strains using GC and GCxGC with High Performance TOFMSPoster
The Pegasus BT 4D facilitates fast and confident cannabis product “fingerprinting” through enhanced two-dimensional chromatographic resolution and high performance TOFMS.
Robust compound identification was achieved through spectral similarity searches of large, well-established databases, mass D determinations, and retention index filtering.
Knockout of microRNAs Using the CRISPR-Cas9 System with Paired Synthetic crRNAsPoster
We utilized paired synthetic crRNAs coupled with our synthetic tracrRNA in cells transduced with lentiviral Cas9 to perform a functional knockout on hsa-miR-221. This three-part system (crRNA, tracrRNA and Cas9) has demonstrated efficient gene editing when used with only one guide RNA, but the goal was to use two crRNAs to remove the entire stem-loop.READ MORE
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