Deciphering Regulatory Mechanisms in M. xanthus Using IsotopicRatio Outlier Analysis (IROA) for Metabolome-wide Quantitation
Poster Jun 15, 2014
Daniel Krug 1,2, Carsten Volz 1, Aiko Barsch 3, Chris Beecher 4, Felice de Jong 4, Rolf Müller 1,2
Myxobacteria are an important source of natural products often exhibiting potent biological activity and unusual modes-of-action . Their genomes - among the largest in prokaryotes - harbour intriguing numbers of secondary metabolite biosynthetic pathways, whereas the small-molecule products for many of these have yet to be discovered . Myxobacteria also devote an exceptionally high proportion of their genetic capacity to regulation, as exemplified by the genome of the model strain Myxococcus xanthus DK1622. Deciphering complex myxobacterial regulatory networks improves our ability to manipulate natural product yields and can facilitate the discovery of novel metabolites. Here, we present a comprehensive metabolomics study using comparative LC-hrMS profiling and IROA to quantify small-molecule readouts in response to induced overexpression of a transcriptional antiterminator in DK1622.
Overcoming the Challenges of Analysing Ionic Polar Pesticides in FoodPoster
Here, the novel application of the Waters’ Torus DEA column is presented, showing the improved performance of a UPLC-MS/MS method for the underivatised
analysis of glyphosate and a selection of other anionic pesticides.
LC-SWATH/MS Metabolomics Platform with Hyphenation of Extraction for the Analysis of Polar and Non-polar Metabolites in Biological SamplesPoster
Automated robotic sample preparation with hyphenated dual LC-SWATH/MS analysis for lipids and polar metabolites.
Validation of an Image-Based 3D Natural Killer Cell Mediated Cytotoxicity AssayPoster
Here we describe a novel 3D NK cell mediated cytotoxicity (CMC) assay.READ MORE