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Defining off-target cleavage in a pair of Zinc Finger Nucleases
Poster

Defining off-target cleavage in a pair of Zinc Finger Nucleases

Defining off-target cleavage in a pair of Zinc Finger Nucleases
Poster

Defining off-target cleavage in a pair of Zinc Finger Nucleases

Studies on Zinc Finger Nucleases (ZFN) have shown that they can be toxic in organisms. This is potentially due to ZFN cleavage at multiple off-target sites. In applications of ZFNs in human gene therapy, this off-target cleavage is intolerable. We are attempting to develop a procedure to identify these off-target sites in Drosophila. We can then analyze every new ZFN pair for potential off-target cleavage and select and redesign it to work more efficiently.

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