deltaDOT Technology Label Free Intrinsic Imaging (LFII™)
Introduction
UV absorption as a technique to detect and quantify biomolecules is routine in molecular biology. LFII™ is a quantum leap up from this state to one that allows the effective imaging of separating biomolecules in an electrophoretic system.
The current Peregrine system is based on standard capillary electrophoresis (CE) of biomolecules such as DNA and proteins, small molecules and larger entities such as bacteria and viruses. The analytes are generated using standard off the shelf sample preparation kits, but without the addition of any label. Typically the amount of analyte injected is remarkably small, only a few 100 picolitres being normal. The sample is electrophoresed and separated as in any similar CE system.
UV absorption as a technique to detect and quantify biomolecules is routine in molecular biology. LFII™ is a quantum leap up from this state to one that allows the effective imaging of separating biomolecules in an electrophoretic system.
The current Peregrine system is based on standard capillary electrophoresis (CE) of biomolecules such as DNA and proteins, small molecules and larger entities such as bacteria and viruses. The analytes are generated using standard off the shelf sample preparation kits, but without the addition of any label. Typically the amount of analyte injected is remarkably small, only a few 100 picolitres being normal. The sample is electrophoresed and separated as in any similar CE system.
