Design and Evaluation of High Definition Probe for HPV Genotyping Microarray
Poster Sep 19, 2014
Sihn Ae Lee, Ah Reum Park, Inyoung Kim, Ji Hyung Lee, and Jongwon Kim
DNA microarray is one of the most useful tools in the Viral Genome Screening such as Human Papilomavirus(HPV). Using type specific probes of about 30 mer oligonucleotide DNA sequences based upon the L1 region of the HPV genome, we could genotyping the HPV which might be infected in the cervix. In this HPV DNA microarray technology, we used the amplicon of 150 bp of the HPV L1 region. We improved the performance of the type specific probe by triple probe sequence in the amplicon. New probes are composed of three types of sequences in the HPV L1 region. To make hybridization conditions amicable, we select the final probe set under melting temperature requirement. The new probes have shown more sensitive than the single sequence probe about 10 ~ 100 times.
Despite the developments in conventional PCR, the complexity of multiplex Real Time PCR is still limited due to the lack of sufficient detection channels. To achieve high-end multiplexing capacity on standard Real Time PCR machines, Anapa Biotech has developed the MeltPlex® technology (see box on right).READ MORE