Design and Evaluation of High Definition Probe for HPV Genotyping Microarray
Poster Sep 19, 2014
Sihn Ae Lee, Ah Reum Park, Inyoung Kim, Ji Hyung Lee, and Jongwon Kim
DNA microarray is one of the most useful tools in the Viral Genome Screening such as Human Papilomavirus(HPV). Using type specific probes of about 30 mer oligonucleotide DNA sequences based upon the L1 region of the HPV genome, we could genotyping the HPV which might be infected in the cervix. In this HPV DNA microarray technology, we used the amplicon of 150 bp of the HPV L1 region. We improved the performance of the type specific probe by triple probe sequence in the amplicon. New probes are composed of three types of sequences in the HPV L1 region. To make hybridization conditions amicable, we select the final probe set under melting temperature requirement. The new probes have shown more sensitive than the single sequence probe about 10 ~ 100 times.
Despite the developments in conventional PCR, the complexity of multiplex Real Time PCR is still limited due to the lack of sufficient detection channels. To achieve high-end multiplexing capacity on standard Real Time PCR machines, Anapa Biotech has developed the MeltPlex® technology (see box on right).READ MORE
Genome-wide association studies (GWAS) have identified more than 100 genetic loci associated with type 2 diabetes. The majority of these are located in the intergenic or intragenic regions suggesting that the implicated variants may alter chromatin conformation. This, in turn, is likely to influence the expression of nearby or more remotely located genes to alter beta cell function. At present, however, detailed molecular and functional analyses are still lacking for most of these variants. We recently analysed one of these loci and mapped five causal variants in an islet-specific enhancer cluster within the STARD10 gene locus. Here, we aimed to understand how these causal variants influence b-cell function by alteration of the chromatin structure of enhancer clusterREAD MORE