Design and Evaluation of High Definition Probe for HPV Genotyping Microarray
Poster Sep 19, 2014
Sihn Ae Lee, Ah Reum Park, Inyoung Kim, Ji Hyung Lee, and Jongwon Kim
DNA microarray is one of the most useful tools in the Viral Genome Screening such as Human Papilomavirus(HPV). Using type specific probes of about 30 mer oligonucleotide DNA sequences based upon the L1 region of the HPV genome, we could genotyping the HPV which might be infected in the cervix. In this HPV DNA microarray technology, we used the amplicon of 150 bp of the HPV L1 region. We improved the performance of the type specific probe by triple probe sequence in the amplicon. New probes are composed of three types of sequences in the HPV L1 region. To make hybridization conditions amicable, we select the final probe set under melting temperature requirement. The new probes have shown more sensitive than the single sequence probe about 10 ~ 100 times.
Inhibition of The Auto-inflammation Suppressor Protein ISG15 Triggers Preeclampsia by Blocking Trophoblast Migration and InvasionPoster
In summary, ISG15 expression levels are crucial for trophoblast morphology and function (migration/invasion). By blocking trophoblast invasion, reduced ISG15 levels could contribute to impaired spiral artery transformation that reduces utero-placental blood flow in preeclampsia. Thus, agents inducing ISG15 expression are likely to be therapeutic in preeclampsia.
An Emerging Phenotype of Partial RAG 1/2 Deficiency Among Young Children with Autoimmunity and Viral InfectionsPoster
We describe the natural history of a cohort of 12 patients with confirmed partial RAG1/2 mutations and autoimmunity at a young age. We were seeking the link between viral infections and autoimmunity and tested candidate biomarkers that may reflect the underlying RAG1/2 protein deficiency.READ MORE
Severe Combined Immunodeficiency (SCID) caused by thymic aplasia due to a mutation in TBX1Poster
We report one of the first cases of TBX1 haploinsufficiency causing complete thymic aplasia and SCID.READ MORE