Design and Functional Analysis of ssDNA Directed Assembly in Protein Array
Poster Feb 01, 2007
Ng Jin Kiata, Parayil Kumaran Ajikumara, Tang Yew Chunga, Lee Jim Yanga, Gregory Stephanopoulosa and Too Heng-Phona
In the post genomic era, the characterization of complex cellular functions requires the large scale analysis of the proteome. Antibody microarray technology has therefore become an invaluable tool in understanding the level of protein expression as well as protein-protein interaction in a high throughput manner. However, current fabrication approach of antibody microarray often results in the loss of antibody functionalities after immobilization onto the substrate.
The present poster discusses the fabrication and evaluation of a new platform called “Spatially addressable protein array” (SAPA). By exploring the specificity of DNA hybridization, ssDNA-antibody conjugates would capture the antigen from complex biological samples in milieu and spatially addressed to specific location on the oligo array for detection.
Such approach allows the protein-analyte interaction to take place in a solution phase and reduce the loss of antibody functionality due to unfavorable surface protein interactions. Further optimization has been done by investigating surface chemistry, non-specific protein adsorption and facile preparation of the ssDNA-conjugated antibody. Experimental studies have shown that the platform is able to detect samples at pM scale and it could be fine-tuned to achieve an optimal system for solving biological problems.
Inhibition of The Auto-inflammation Suppressor Protein ISG15 Triggers Preeclampsia by Blocking Trophoblast Migration and InvasionPoster
In summary, ISG15 expression levels are crucial for trophoblast morphology and function (migration/invasion). By blocking trophoblast invasion, reduced ISG15 levels could contribute to impaired spiral artery transformation that reduces utero-placental blood flow in preeclampsia. Thus, agents inducing ISG15 expression are likely to be therapeutic in preeclampsia.
An Emerging Phenotype of Partial RAG 1/2 Deficiency Among Young Children with Autoimmunity and Viral InfectionsPoster
We describe the natural history of a cohort of 12 patients with confirmed partial RAG1/2 mutations and autoimmunity at a young age. We were seeking the link between viral infections and autoimmunity and tested candidate biomarkers that may reflect the underlying RAG1/2 protein deficiency.READ MORE
Immunotherapy on Chip: “Under the Microscope” Drug-induced Modulation of the Immune ResponsePoster
The immune system is a striking example of an integrated information system, engaged in coordinated host-protective activities. Organs-on-chip approach (OOC) models allow the direct simultaneous observation of hundreds of different cells, moving, interacting and responding to signals coming from the microenvironment nearby, that give access to a number of parameters describing the system that must be properly measured and elaborated.READ MORE