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Detection of 17 Targets in a Single PCR Tube by a Novel MeltPlex® Probe System Combining Melting Curves and Taqman Probes

Poster   Sep 05, 2018

 
Detection of 17 Targets in a Single PCR Tube by a Novel MeltPlex® Probe System Combining Melting Curves and Taqman Probes
 
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by Søren M Echwald.

 
 

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License

 
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Characterization of a Type 2 diabetes-associated islet-specific enhancer cluster in STARD10 by genome editing of EndoC-βH1 cells

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Genome-wide association studies (GWAS) have identified more than 100 genetic loci associated with type 2 diabetes. The majority of these are located in the intergenic or intragenic regions suggesting that the implicated variants may alter chromatin conformation. This, in turn, is likely to influence the expression of nearby or more remotely located genes to alter beta cell function. At present, however, detailed molecular and functional analyses are still lacking for most of these variants. We recently analysed one of these loci and mapped five causal variants in an islet-specific enhancer cluster within the STARD10 gene locus. Here, we aimed to understand how these causal variants influence b-cell function by alteration of the chromatin structure of enhancer cluster

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