Proprietary Novel Fluorescent Proteins (NFP) from BD Biosciences Clontech include fluorescent proteins cloned from reef corals and the jellyfish Aequorea coerulescens. Fluorescent emissions of distinct proteins from this portfolio were analysed by the Acumen Explorer. Single and mixed populations of cells expressing NFP genes were detected in addition to the measurement of proteins linked to different colored NFP’s localized to cell compartments. The combination of these technologies provides an excellent tool for the investigation of functional genomic applications.
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Despite the developments in conventional PCR, the complexity of multiplex Real Time PCR is still limited due to the lack of sufficient detection channels. To achieve high-end multiplexing capacity on standard Real Time PCR machines, Anapa Biotech has developed the MeltPlex® technology (see box on right).