DETERMINATION OF HOMOCYSTEINE THIOLACTONE IN URINE BY FIELD AMPLIFIED SAMPLE INJECTION AND SWEEPING MEKC METHOD WITH UV DETECTION
Poster Aug 25, 2014
Paulina Furmaniak, Pawel Kubalczyk, Kamila Borowczyk, Rafal Glowacki
Homocysteine-thiolactone (HTL) is a cyclic thioester of homocysteine, which is synthesized by methionyl-tRNA synthetase, that mistakenly activates cyclization of homocysteine. HTL is highly reactive and readily reacts with ε-amino groups of protein lysine residues in a process called N-homocysteinylation. This reaction alters the proteins structure and function and contributes to the emergence of many diseases. Because of its low concentration in biological fluids it is important to choose the determination technique outstanding high sensitivity and low operating cost.
Capillary electrophoresis is a modern analytical technique based on differences in the electrophoretic mobility of ions in an electric field. This technique has a high resolution, short analysis time and low costs associated with low use of reagents and samples. It allows accurate both qualitative and quantitative determinations. A very important role in the process of increasing the sensitivity in capillary electrophoresis, plays FASI-sweeping MEKC stacking technique.
We present novel CE method utilizing FASI-sweeping MEKC stacking for the determination of HTL in human urine. Initially, the capillary was conditioned with 0.1M pH 6 phosphate buffer and then, the sample (pH 5.5) was electrokinetically introduced into the CE system. The analysis were performed at a voltage of 20 kV using 0.1M pH 4.75 phosphate buffer with the addition of surfactant and acetonitrile. Application of FASI-sweeping MEKC stacking technique caused sweeping of the analyte, allowing the determination of HTL up to 100 nM. All separations were performed at 25 C with detector set to measure peaks at 240 nm.
Hepatocellular Carcinoma with Small Cell Histology: A Rare Mimic of Metastatic Tumors Involving the LiverPoster
Two cases of hepatocellular carcinoma (HCC) with small cell histology, a rare morphologic variant of HCC, are presented.READ MORE
Severe Combined Immunodeficiency (SCID) caused by thymic aplasia due to a mutation in TBX1Poster
We report one of the first cases of TBX1 haploinsufficiency causing complete thymic aplasia and SCID.READ MORE
Analysis of the Effect of Aggregated β-Amyloid on Cellular Signaling Pathways Critical for Memory in Alzheimer’s DiseasePoster
Here we evaluate the ability to detect changes in phosphorylation levels of ERK and CREB following treatment with Aβ using the SH-SY5Y neuroblastoma cell line.READ MORE
Comments | 0 ADD COMMENT
International Conference on Analytical and Bio Analytical Techniques
Oct 31 - Nov 01, 2018
6th International Conference on Neurodegenerative Disorders and Stroke
Oct 22 - Oct 23, 2018
16th Annual Conference on Laboratory Medicine and Pathology
Sep 17 - Sep 18, 2018