Determination of the Autoantibody Repertoire in Autoimmune Diseases with Factor Protein Arrays – A new Approach to Personalized Therapies
Poster Apr 05, 2007
A. Lueking, C. Gutjahr, V. Trappe, M. Ciupke, A. Kowald, K. Schulte, S. Cleves, T. Niehues, S. Schimrigk, M. Schneider, H. E. Meyer, Stefan Müllner and Jens Beator
Recently, we have presented a strategy to identify new marker molecules characteristic for Alopecia areata by combining protein microarray technology with the use of large cDNA expression libraries (Lueking et al., 2005). This strategy is now applied to Juvenile idiopathic Arthritis (JIA), Rheumatoid Arthritis (RA) and Multiple Sclerosis (MS) in a BMBF-funded project.
When there is a need to quickly analyze samples using a number of different PCR assays, it is likely that optimal conditions for each assay will not be the same. First, different assays often will require different annealing temperatures for their primers. In addition, amplicons may be designed to be of different lengths and therefore require varying durations of the extension step.READ MORE