Development and optimization of a procedure for extracting proteins from fixed archival tissues and assessment of its potential for biomarker discovery in human and veterinary medicine
Poster Dec 18, 2013
Alessandro Tanca, Daniela Pagnozzi, Sergio Uzzau, Maria Filippa Addis
Fixed tissue repositories are an ideal information mine for biomarker discovery, with their vast collections of diseased tissues and associated clinical records describing diagnosis, prognosis, therapy, and outcome. Unfortunately, fixation hampers protein extraction, posing considerable limitations to proteomic investigations. In this scenario, and building on the existing literature, several methods were combined and refined to enable extraction of high-quality, full-length proteins from the fixed specimens, leading to the definition of an optimized protocol with unprecedented performances. In order to test its ability to provide meaningful results in terms of differential disease traits and enable biomarker discovery, the optimized method was applied to sample repositories stored in pathology departments. Specifically, diseased human, canine and sheep tissues were retrieved and subjected to the optimized extraction procedure, followed by established proteomic pipelines consisting of electrophoresis, image analysis, mass spectrometry, and data processing by ontology and pathway analysis. The method enabled gel-based proteomic investigations of archival tissues, performing better than commercially available systems. Differential proteins were successfully identified in all cases, and these were clearly related to the disease biology. In conclusion, biomarker discovery strategies based on proteomic investigations can be successfully performed on fixed archival tissues by means of this optimized extraction protocol.
Identifying Non-Intentionally Added Substances (NIAS) in Food Contact MaterialsPoster
Concern about the safety of food products has increased dramatically with intentionally and non-intentionally added substances (NIAS) used in packaging being of particular interest. APGC and high resolution MS together with a workflow driven process was used to identify unknown compounds in packaging.READ MORE
Human exposure to cyanotoxins: Exploring in vitro detoxification using atmospheric cold plasma treatment to protect human healthPoster
Recently, there have been increases in freshwater harmful algal blooms. There is therefore a need to try and effectively detoxify drinking water contaminated with cyanotoxins to safeguard human health by use of new and novel techniques.READ MORE
Psychiatric Risk Gene Cacna1c and Early Life Stress: Potential Gene-Environment interactions?Poster
Early life stress (ELS) is highly associated with development of psychopathology
and mood disorders in adulthood. Genetic studies have identified variation in the gene calcium voltage-gated channel subunit alpha1C (CACNA1C) to increase risk for several psychiatric disorders. This poster assessed the expression of Cacna1c following prepubertal stress.