Development of a Homogenous RSV Neutralization Assay on the Mirrorball Fluorescence Cytometer: A Simple, Safe and Robust Approach for High-throughput Screening
Poster Jul 08, 2016
Anne F. Hammerstein and Lucy Chammas
Human respiratory syncytial virus (RSV) is a syncytial virus that can cause serious respiratory tract infections, especially in infants and young children.
To date there is no licensed vaccine against RSV infection and the only preventative treatment option is the neutralizing anitbody palivizumab, which is given to high risk infants by passive administration. Therefore there is a strong drive to identify novel RSV-neutralizing antibodies for therapeutic/preventative applications.
Here we present the development and implementation of a simple RSV-neutralization assay on TTP Labtech’s mirrorball fluorescence cytometer.
When there is a need to quickly analyze samples using a number of different PCR assays, it is likely that optimal conditions for each assay will not be the same. First, different assays often will require different annealing temperatures for their primers. In addition, amplicons may be designed to be of different lengths and therefore require varying durations of the extension step.READ MORE