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Development of a Novel Xeno-free Medium for Feeder-free Culture of Human Stem Cells

Poster   Mar 20, 2015

 
Development of a Novel Xeno-free Medium for Feeder-free Culture of Human Stem Cells
 
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Annand R; Okuda Y; Inamura M

 
 

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License

 
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Manage impurity data in pharmaceutical development more effectively in a single informatics solution. Assemble all relevant analytical data with chemical information for real-time access to project information by the entire team.

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The nuclear receptors pregnane X receptor (PXR) and constitutive androstane receptor (CAR) are closely related transcription factors that regulate the expression of phase I (cytochrome P450s), phase II metabolizing enzymes and transporter genes in response to xenobiotics, including prescription drugs.

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Regulatory T-Cells (Tregs) Within Bone Marrow-Derived Stem Cells (BMSCs) Actively Confer Immunomodulatory and Neuroprotective Effects Against Stroke

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We found a distinct subpopulation of Tregs within BMSCs. Tregs and BMSCs in co-culture conferred neuroprotection that varied in a dose-dependent manner. Tregs minimized stem cell production of IL-6, a pro-inflammatory cytokine, and inhibited BMSC secretion of FGF-beta, a cytokine related to BMSC proliferation and differentiation. The ratio of Tregs found natively in BMSCs is optimally adapted to provide the maximum neuroprotective benefit of stem cell treatment after ischemic stroke.

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