Development of a Nucleic Acid-based Amplification Test as Point-of-Care Rapid Test Detecting Sexually-transmitted Diseases for Home-care
Poster Oct 13, 2015
Vicky Tröger, Anja Scherber, Dirk Kuhlmeier, Indrek Tulp, Marko Lehes, Steffen Howitz, Toni Bohatzsch, Tamas Pardy
In Europe, 350.000 new sexual transmitted infections (STI) caused by Chlamydia types have been registered in 2009 . Approximately 70 % of infections with Chlamydia trachomatis run an asymptomatic course and therefore remain undetected. This can result in severe complications such as chronic diseases, infertility, complications in pregnancy and cancer [2, 3]. Current detection systems commercially available are based on serological methods. However, existing detection strategies have a number of disadvantages including the high market price, delayed diagnostics in the course of infection, false-negative results. Therefore a new, innovative detection system for home-care devices is a rising demand.
We present a microfluidic test system which connects two methods with each other. It combines a high specific isothermal nucleic acid amplification technology with a detection on a simple lateral flow strip.
The microfluidic chip is completed with a non-instrumented mirco-heating solution by using chemical exothermal reactions. As a whole, this nucleic quick test cartridge for Chlamydia trachomatis screening is a promising tool in home-care diagnosis.
When there is a need to quickly analyze samples using a number of different PCR assays, it is likely that optimal conditions for each assay will not be the same. First, different assays often will require different annealing temperatures for their primers. In addition, amplicons may be designed to be of different lengths and therefore require varying durations of the extension step.READ MORE