Development of an Automated Platform for HT Cloning and Expression
Poster Jun 22, 2012
Stefano Bonacci; Sara Iozzi; Scilla Buccato; Manuele Martinelli; John Telford; Domenico Maione; Roberto Petracca
• More and more frequently, research projects in fields like structural biology, protein-protein interaction and vaccinology require cloning, expression and purification of a high number of proteins.
• In the Cloning and Expression Unit at Novartis V&D in Siena we have long been involved in Reverse Vaccinology projects, which requires the production and screening of hundreds of antigens with the aim to select the best vaccine candidates (Rinaudo et al., 2009). Then we decided to exploit automation in order to speed up and potentiate our high throughput (HT) procedures, even under the consideration of the significant advances made in the field over the latter years and successfully implemented to molecular biology techniques (Lien and Lee, 2010; Hughes et al., 2011).
When there is a need to quickly analyze samples using a number of different PCR assays, it is likely that optimal conditions for each assay will not be the same. First, different assays often will require different annealing temperatures for their primers. In addition, amplicons may be designed to be of different lengths and therefore require varying durations of the extension step.READ MORE