Development of an Integrated Paper-based Molecular Diagnostic Platform
Poster Mar 15, 2016
Manoharanehru Branavan, Wamadeva Balachandran
Point-of-Care testing (POCT) devices have received immense attraction due to its simplicity, ease of use and swiftness. Microfluidic paper-based analytical devices (µPADs) are directly aimed for POC testing. Molecular diagnostics result in a more specific and sensitive assay than immunoassays with much smaller window time to diagnosis. Realizing molecular diagnostics on paper-based devices is a challenge that has been little addressed. A chitosan functionalized paper-based nucleic acid extraction method, isothermal amplification of Chlamydia trachomatis on paper-based devices, and endpoint detection on a nucleic acid lateral flow test strips are presented here. In addition, a proprietary integrated paper-based diagnostic device developed to perform lysate-in-to-answer-out in less than an hour with a limit of detection of 0.2 copies/µL and on going studies to realise a complete system suitable for resource limited settings are also presented. The integrated device is capable of performing affordable, sensitive and specific, and equipment free molecular diagnostic assays in just 6 easy sequential processes.
When there is a need to quickly analyze samples using a number of different PCR assays, it is likely that optimal conditions for each assay will not be the same. First, different assays often will require different annealing temperatures for their primers. In addition, amplicons may be designed to be of different lengths and therefore require varying durations of the extension step.READ MORE