Development of low Cost High Throughput miRNA Screening
Poster Sep 20, 2005
JF Laes, P Lienard, C Sequaris, I Gonze and JP Detiffe
We have developed a low cost high throughput method to quantify the expression of these miRNAs via classictotal RNA extraction without interference from genomic DNA. More than 150 human and more than 100 mouse miRNAs have been validated using as little as 100ng total RNA per miRNA. This method provides accurate sensitive miRNA expression profiles which can be used to identify specific miRNA signatures associated with tissues, biological processes or diseases.
Despite the developments in conventional PCR, the complexity of multiplex Real Time PCR is still limited due to the lack of sufficient detection channels. To achieve high-end multiplexing capacity on standard Real Time PCR machines, Anapa Biotech has developed the MeltPlex® technology (see box on right).READ MORE