DHPLC Technology as a High-throughput Detection of Mutations in a Durum Wheat TILLING Population
Poster Feb 02, 2015
Colasuonno P.1, Incerti O. 1, Lozito M.L. 1, Sbalzarini M. 2, Zaccagna P. 2, Papadimitriou S. 2, Blanco A. 1, Gadaleta A. 1
Durum wheat (Triticum turgidum ssp. durum) has a tetraploid genome (AABB genome) with genes in two homoeologous copies, a high proportion of repetitive DNA regions and a low gene density. Current tools for the discovery of sequence alterations in target genes rely on reverse genetic approaches such as the TILLING (Targeting Induced Local Lesions IN Genomes) technique. TILLING is based on the detection of chemical induced mutations in heteroduplex mainly using endonuclease (CelI enzyme), standard gel electrophoresis, fluorescent capillary electrophoresis and/or LI-COR techniques. Alternatively, DHPLC approach reveals the presence of genetic variation by the differential retention of the homo- and heteroduplex DNA molecules on ion-pair reversed-phase high-pressure liquid chromatography supported under partial denaturing conditions.
Psychiatric Risk Gene Cacna1c and Early Life Stress: Potential Gene-Environment interactions?Poster
Early life stress (ELS) is highly associated with development of psychopathology
and mood disorders in adulthood. Genetic studies have identified variation in the gene calcium voltage-gated channel subunit alpha1C (CACNA1C) to increase risk for several psychiatric disorders. This poster assessed the expression of Cacna1c following prepubertal stress.
Performance of the D5000 and High Sensitivity D5000 ScreenTape Assays for the 4200 TapeStation SystemPoster
Here, we focus on quantification, sizing, and sensitivity of both D5000 ScreenTape assays.READ MORE
Applications of chemically modified synthetic guide RNA for CRISPR-Cas9 genome editingPoster
Our results indicate that MS modifications are required for experiments with co-electroporation of Cas9 mRNA and synthetic gRNA, yet have no impact on editing efficiency when delivered with lipid-based transfection reagents.READ MORE