Diagnosis of Aortic Aneurysm from Gene Expression Profiling of Peripheral Blood
Poster Feb 01, 2007
Catalin Barbacioru,Yulei Wang, Dov Shiffman, Olga Iakoubova, Sriram Balasubramanian, Julie Blake, John Elefteriades and Raymond Samaha
We report in this study that gene expression profiles of peripheral blood cells may allow early detection and diagnosis of aortic aneurysm. Gene expression profiles of peripheral blood samples collected from 58 individuals diagnosed with thoracic aortic aneurysm (cases) and 36 normal individuals (controls) were analyzed using the Applied Biosystems Expression Array Systems and Human Genome Survey Microarrays.
When there is a need to quickly analyze samples using a number of different PCR assays, it is likely that optimal conditions for each assay will not be the same. First, different assays often will require different annealing temperatures for their primers. In addition, amplicons may be designed to be of different lengths and therefore require varying durations of the extension step.READ MORE