Digital Dispensing for Direct Dilution: New Flexibility in Dose-Response Analyses
Poster Feb 21, 2013
Jeff Nielsen, Ken Ward, Christie Dudenhoefer, David Ochs, Joshua Yu, and Kevin
HP has been the worldwide leader in inkjet technology for 25 years with HP printers reliably dispensing billions of picoliter droplets per day. The HP T8 Dispensehead is built on a silicon wafer using HP inkjet photolithography to enable precise, accurate drop generation and placement. The single-use dispensehead utilizes 22 high-frequency nozzles so that picoliter to microliter volumes are dispensed in under a second.
Direct dilution using HP picoliter digital dispensing technology was shown to be a replacement for serial dilution. Compound usage can be reduced by 90%, while reliability and speed are improved with simplified workflows. Non-contact dispensing of droplets directly into assay wells make it practical to routinely perform drug-drug interaction experiments.
This non-contact digital dispensing system frees the researcher from the constraints of analog-based dispensing methodologies, enabling complicated experiments that have not been practical with manual or robotic serial dilution methods.
We utilized paired synthetic crRNAs coupled with our synthetic tracrRNA in cells transduced with lentiviral Cas9 to perform a functional knockout on hsa-miR-221. This three-part system (crRNA, tracrRNA and Cas9) has demonstrated efficient gene editing when used with only one guide RNA, but the goal was to use two crRNAs to remove the entire stem-loop.READ MORE
During early drug discovery, the study of metabolism plays an essential role in determining which drug candidates move forward into development and later stages. As an alternative to traditional Data Dependent Acquisition (DDA), the use of MSE/All Ions Fragmentation (AIF) has become common in metabolite identification workflows for the analysis of metabolic hot spots. Here we present a solution for analysis of MSE/AlF in metID studies.READ MORE