Digital Dispensing for Direct Dilution: New Flexibility in Dose-Response Analyses
Poster Feb 21, 2013
Jeff Nielsen, Ken Ward, Christie Dudenhoefer, David Ochs, Joshua Yu, and Kevin
HP has been the worldwide leader in inkjet technology for 25 years with HP printers reliably dispensing billions of picoliter droplets per day. The HP T8 Dispensehead is built on a silicon wafer using HP inkjet photolithography to enable precise, accurate drop generation and placement. The single-use dispensehead utilizes 22 high-frequency nozzles so that picoliter to microliter volumes are dispensed in under a second.
Direct dilution using HP picoliter digital dispensing technology was shown to be a replacement for serial dilution. Compound usage can be reduced by 90%, while reliability and speed are improved with simplified workflows. Non-contact dispensing of droplets directly into assay wells make it practical to routinely perform drug-drug interaction experiments.
This non-contact digital dispensing system frees the researcher from the constraints of analog-based dispensing methodologies, enabling complicated experiments that have not been practical with manual or robotic serial dilution methods.
High Throughput Rheological Characterization of Small Volume Biopharmaceutical FormulationsPoster
Importance of assessing viscosity fingerprints of small molecules.READ MORE
Applications of chemically modified synthetic guide RNA for CRISPR-Cas9 genome editingPoster
Our results indicate that MS modifications are required for experiments with co-electroporation of Cas9 mRNA and synthetic gRNA, yet have no impact on editing efficiency when delivered with lipid-based transfection reagents.READ MORE
Novel lead optimization strategy of BACE I inhibitors for the treatment of Alzheimer’s disease by Quantitative Structure-Activity Relationship (QSAR) and Physiologically-Based Pharmacokinetics (PBPK) modelingPoster
In this study, we introduce an innovative in silico-based high throughput lead optimization strategy with QSAR and PBPK modelings using StarDrop™, ADMET predictor® and GastroPlus®.READ MORE