Digital microphotographic atlas of the adult zebrafish cerebellum
Poster Apr 05, 2014
Kristen Lee, Megan Landy, Ashton Cline, Christopher Corbo, and Zoltan Fulop
Sagittal, coronal and horizontal, one micron thick plastic (semithin) sections of adult zebrafish (Danio rerio) brain were cut serially, using an ultramicrotome, mounted on glass slides and stained with toluidine blue. Each cerebellar region of the brain sections was photographed in 40X using a computer assisted Olympus light microscope, montaged via Photoshop and arranged in series. Roughly 10 slices from each sectional orientation were selected. The resulting pictures are presented in pairs, so the three dimensional anatomical relationships of the zebrafish cerebellum could be visualized by preparing all three sections together. The final product resulted in a digital microphotographic atlas of the adult zebrafish cerebellum, which is a very useful tool to researchers alike.
We found a distinct subpopulation of Tregs within BMSCs. Tregs and BMSCs in co-culture conferred neuroprotection that varied in a dose-dependent manner. Tregs minimized stem cell production of IL-6, a pro-inflammatory cytokine, and inhibited BMSC secretion of FGF-beta, a cytokine related to BMSC proliferation and differentiation. The ratio of Tregs found natively in BMSCs is optimally adapted to provide the maximum neuroprotective benefit of stem cell treatment after ischemic stroke.READ MORE