Direct On-chip Storage and Release of Liquid Reagents for Diagnostic Lab-on-a-Chip Devices
Poster Mar 10, 2014
Daniel Czurratis*, Thomas Brettschneider*, Yvonne Beyl*, Sven Zinober*, Franz Lärmer*, Roland Zengerle**
On-chip storage of liquid reagents is one of the major challenges for the commercialization of polymer-based LoC devices. The key advantages of on-chip reagent storage are: ready for operation, fully automated processing, ease of use, reduction of contamination and transportation risks. In order to guarantee long-term storage of liquids several concepts have been presented , for example reagent storage in glass ampoules  or stick packs . Since these concepts implicate manufacturing complexity, direct on-chip storage of liquid reagents in reservoirs is preferable. The solution is to provide the required reagents in reservoirs made of high barrier polymers  (e.g. PP, PE, COP, COC), which are sealed with a standard barrier film by hot embossing. The barrier film includes an aluminium layer as diffusion barrier, which is structured by laser ablation. This installs rated brake points, without affecting the barrier properties where needed. To release the reagents on demand, a flexible membrane is actuated through pneumatic pressure. The expanding membrane breaks up the rated break points and enables efficient cleaning of the reservoirs for further microfluidic processing. This on-chip reagent storage concept is a big step forward commercialization of LoC-applications.
The immune system is a striking example of an integrated information system, engaged in coordinated host-protective activities. Organs-on-chip approach (OOC) models allow the direct simultaneous observation of hundreds of different cells, moving, interacting and responding to signals coming from the microenvironment nearby, that give access to a number of parameters describing the system that must be properly measured and elaborated.READ MORE