Discovery of Conserved Domains for Development of Functional Markers and Identification of microRNAs from Drought Responsive Expressed Sequence Tags (ESTs) in Chickpea (Cicer arietinum L.)
Poster Oct 18, 2012
Mandape M, Saikumar K, Dudhe MY, Kumar J, Meena HP, Chauhan J, Afroz A
Domains can be thought of as distinct functional and structural units of protein. We have analyzed 5885 drought responsive expressed sequence tags (ESTs) for the identification of conserved domains and microRNAs in chickpea. The occurrence of conserved domains, super families and multi domains having putative functions like insect pest resistance, improved quality parameters, resistance to abiotic stresses other than drought viz., temperature, salinity, evolutionary functions and physiogenic nature have been identified. Identified conserved domains or gene families from the drought responsive ESTs could be utilized for the identification of gene-targeted markers and for development of functional markers to accelerate marker assisted breeding in chickpea. Based on homology and secondary structure analysis potential miRNA targets have been found for newly identified chickpea conserved miRNAs. These target genes included number of transcription factors that control plant growth, development and metabolic enzymes, as well as other genes involved in stress response.
Despite the developments in conventional PCR, the complexity of multiplex Real Time PCR is still limited due to the lack of sufficient detection channels. To achieve high-end multiplexing capacity on standard Real Time PCR machines, Anapa Biotech has developed the MeltPlex® technology (see box on right).READ MORE
Genome-wide association studies (GWAS) have identified more than 100 genetic loci associated with type 2 diabetes. The majority of these are located in the intergenic or intragenic regions suggesting that the implicated variants may alter chromatin conformation. This, in turn, is likely to influence the expression of nearby or more remotely located genes to alter beta cell function. At present, however, detailed molecular and functional analyses are still lacking for most of these variants. We recently analysed one of these loci and mapped five causal variants in an islet-specific enhancer cluster within the STARD10 gene locus. Here, we aimed to understand how these causal variants influence b-cell function by alteration of the chromatin structure of enhancer clusterREAD MORE
Early life stress (ELS) is highly associated with development of psychopathology
and mood disorders in adulthood. Genetic studies have identified variation in the gene calcium voltage-gated channel subunit alpha1C (CACNA1C) to increase risk for several psychiatric disorders. This poster assessed the expression of Cacna1c following prepubertal stress.