DNA Methylation as a Marker of the Intra Uterine Environment
Poster May 05, 2011
Carolyn Banister, Devin Koestler, Matthew A. Maccani, E. Andres Houseman, James F. Padbury and Carmen J. Marsit
Using the Illumina Infinium Human Methylation27 BeadChip array, we examined genome-wide DNA methylation patterns in 206 term human placenta samples. Semi-supervised recursive-partitioned mixture modelling was employed to analyze data from a training series of placenta samples and identify methylation profiles associated with aberrant fetal growth.
A number of CpG loci were found to effectively differentially classify intrauterine growth restriction (IUGR) and small for gestational age (SGA) placentas from appropriate for gestation alage (AGA) placentas, and these associations were validated in a masked testing series of samples. Our work demonstrated that patterns of DNA methylation in human placenta are reliably and significantly associated with infant growth and serve as a proof of principle that methylation status in the human term placenta can function as a marker for the intrauterine environment.
Despite the developments in conventional PCR, the complexity of multiplex Real Time PCR is still limited due to the lack of sufficient detection channels. To achieve high-end multiplexing capacity on standard Real Time PCR machines, Anapa Biotech has developed the MeltPlex® technology (see box on right).READ MORE
Genome-wide association studies (GWAS) have identified more than 100 genetic loci associated with type 2 diabetes. The majority of these are located in the intergenic or intragenic regions suggesting that the implicated variants may alter chromatin conformation. This, in turn, is likely to influence the expression of nearby or more remotely located genes to alter beta cell function. At present, however, detailed molecular and functional analyses are still lacking for most of these variants. We recently analysed one of these loci and mapped five causal variants in an islet-specific enhancer cluster within the STARD10 gene locus. Here, we aimed to understand how these causal variants influence b-cell function by alteration of the chromatin structure of enhancer clusterREAD MORE
2nd International Conference on Pharmaceutical Research & Innovations in Pharma Industry
May 30 - May 31, 2019