Does the increase of exosomal microRNAs reflect an activated immune system in melanoma?
Poster Dec 12, 2013
Nina Koliha, Florian S. Dreyer , Jochen Dindorf , Andreas Bosio, Andreas S. Baur , and Stefan Wild
One of the new classes of potential cancer biomarkers are microRNAs. MicroRNAs are non-coding RNAs that suppress the translation of their target mRNAs by binding to the 3’ untranslated region³. On the one hand, melanoma-derived exosomes are discussed as vesicles for degradation of anti-tumor microRNAs. On the other hand, exosomal microRNAs might be active in recipient cells4, e.g., by repressing anti-tumorigenic immune responses. To investigate these possibilities, we profiled the microRNA content of exosomes from melanoma cell lines and plasma of melanoma patients. Informed consent was collected according to guidelines for medical and research ethics.
Primary Human Hepatocyte (PHH) culture provides the closest in vitro model to human liver that can produce a metabolic profile of a given drug very similar to that found in vivo. Recently we have developed an easy-to-assemble user-friendly in vitro Primary Human Hepatocyte (PHH) 3D-spheroid modelREAD MORE