Droplet Microfluidics for 3D Epithelial Cell Culture
Poster Mar 25, 2014
Monika Dolega (Pyzalska), Xavier Gidrol, Nathalie Picollet-D’hahan
We have developed a microfluidic system which delivers highly-controlled conditions for the seeding and growth of polarized cellular spheres, or acini, within matrigel beads. We use high-throughput flow handling to change culture conditions and to move acini through flow-analysis devices on an acini-by-acini basis. Using these optimized parameters we can now design RNAi screens that will identify genes important for the regulation of acini formation in a 3D environment.
Despite the developments in conventional PCR, the complexity of multiplex Real Time PCR is still limited due to the lack of sufficient detection channels. To achieve high-end multiplexing capacity on standard Real Time PCR machines, Anapa Biotech has developed the MeltPlex® technology (see box on right).READ MORE