Droplet Microfluidics for 3D Epithelial Cell Culture
Poster Mar 25, 2014
Monika Dolega (Pyzalska), Xavier Gidrol, Nathalie Picollet-D’hahan
We have developed a microfluidic system which delivers highly-controlled conditions for the seeding and growth of polarized cellular spheres, or acini, within matrigel beads. We use high-throughput flow handling to change culture conditions and to move acini through flow-analysis devices on an acini-by-acini basis. Using these optimized parameters we can now design RNAi screens that will identify genes important for the regulation of acini formation in a 3D environment.
Primary Human Hepatocyte (PHH) culture provides the closest in vitro model to human liver that can produce a metabolic profile of a given drug very similar to that found in vivo. Recently we have developed an easy-to-assemble user-friendly in vitro Primary Human Hepatocyte (PHH) 3D-spheroid modelREAD MORE