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Droplet Microfluidics for 3D Epithelial Cell Culture
Poster

Droplet Microfluidics for 3D Epithelial Cell Culture

Droplet Microfluidics for 3D Epithelial Cell Culture
Poster

Droplet Microfluidics for 3D Epithelial Cell Culture

We have developed a microfluidic system which delivers highly-controlled conditions for the seeding and growth of polarized cellular spheres, or acini, within matrigel beads. We use high-throughput flow handling to change culture conditions and to move acini through flow-analysis devices on an acini-by-acini basis. Using these optimized parameters we can now design RNAi screens that will identify genes important for the regulation of acini formation in a 3D environment.
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